Mahmudi Mahmudi
Fahimeh Mohammadghasemi
Farideh Hosseini
Zahra Atrkar Roshan

Background: Acetylsalicylic acid (ASA) is a non-steroidal anti inflammatory drug, causes reproductive failure in human males or animals. The objective of this study was to evaluate correlation between sperm chromatin dispersion(SCD) test and sex hormones in adult mice treated with low doses of acetylsalicylic acid.Methods: A total of 49 adult male mice were equally divided into seven groups. Group 1 (control) received no drug. Group 2 (sham) received vehicle. Groups 3,4,5,6 and 7 received 0.05,0.1, 0.5,1 and 5 mg/kg ASA. All animals were treated orally for 14 days. On day 15, epididymis were removed and evaluations were made by radioimmunoassay (RIA) and SCD test for study of serum testosterone or LH level and sperm’s DNA fragmentation respectively.Result: Serum LH levels were not changed in all groups, however groups 5, 6 showed significantly reduced serum testosterone levels (pConclusion: The results showed that ASA has deleterious effects on sperm’s DNA especially in dose of 5mg/kg. There is a significant correlation between testosterone and sperm’s DNA fragmentation in higher doses of ASA. These effects may be due to testosterone hormone alterations.

Chromatin dispersion test, DNA fragmentation, Sex hormones, Acetylsalicylic acid