Farnam Azad - Department of Biology, Faculty of Science, Urmia university, Urmia, Iran
Gholamreza Najafi - Depatment of Basic Scienses, Facullty of Veternary Medicine, Urmia University, Urmia, Iran
Vahid Nejati - Department of Biology, Faculty of Science, Urmia university, Urmia, Iran
Fatemeh Rahmani - Department of Biology, Faculty of Basic Science, Urmia University, Urmia, Iran

Background: Smoking is a major factor that lead to male infertility. Nicotine as one of the most important hazardous substrates in cigarette is reported to directly impair male fertility and embryo development. The aim of this work is to show whether antifertility agent with protective effect of royal jelly(RJ).Methods: In this study, 36 male balb/c mice weighing 25-30 g were randomly divided into six groups(n=6) .Group 1 received 0.2 normal salin (control), group 2 received 100 mg/kg RJ, group 3 and 4 received 0.5 mg/kg and 1.0 mg/kg of nicotine respectively, group 5 received 0.5 mg/kg of nicotine with 100 mg/kg of RJ, group 6 received 1.0 mg/kg nicotine and 100 mg/kg RJ. All administration were done orally. After gavage for 30 days, fertilizing capacity of epididymal sperm and blastocyst rate of all animal was evaluated following in vitro fertilization.Result: The results showed that fertilization and blastocyst rate decreased significantly (pConclusion: These results suggested that royal jelly may be improved adverse effect of nicotine on fertilization and blastocyst rate and fertilized oocyte.

IVF, Nicotine, Royal jelly, Fertility