S Noori manesh - Department of Microbiology & Genetics, Faculty of Basic science, Islamic Azad University, Rasht Branch, Iran.P .O. Box: ۴۱۳۳۵-۳۵۱۶
T Shafighi - Department. of Microbiology & Genetics, Faculty of Basic science, Islamic Azad University, Rasht Branch, Iran. P .O. Box: ۴۱۳۳۵-۳۵۱۶
G Abdollahpour - Department of Internal medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.P .O. Box: ۱۴۱۵۵- ۶۴۵۳

Introduction: Leptospirosis is a bacterial and zoonotic disease caused by Leptospira species. Guilan province is an endemic region for this disease due to its humid climate with heavy rainfall. The aim of this study was serological diagnosis of leptospirosis in persons referred to Astara Shahid Beheshti hospital.Material and Mathods: Between February and May 2015, 98 random serum samples were collected from persons referred to Astara central laboratory of Shahid Beheshti Hospital All serum samples were serologically tested by microscopic agglutination test(MAT) using live antigens representing Leptospira interrogans serrovars: Gripotyphosa, Icterohaemorrhagiae, Canicola, Hardjo and Pomona.Results: The result of this study showed that of the 98 samples taken from patients, the rate of positive reaction against serovar , Gripotyphosa, and Hardjo was 2%, 1%, and 1%, respectively. The highest serological titre was measured to be1:200.Conclusion: It was concluded that the low prevalence of Leptospira.spp in Astara city could be attributed to the increased awareness of transmission routes of this disease, improved hygiene and sanitation practices, avoidance of contaminated water and mechanization of farming

Astara, leptospirosis, serological diagnosis