Effects of Resveratrol on Sperm Parameters Disorders Induced By Morphine in Mice

Addiction is a problem which has increasingly developed among the various populations throughout the world. Morphine is one of the alkaloids of opium derived from the opium poppy. Resveratrol is a phytoestrogen and antioxidant of the red grape. The main goal is to investigate whether the Resveratrol could inhibit morphine adverse effects on sperm cell viability, count, motility and testis histology and testosterone hormone and nitric oxide levels in mice.In this study, 48 male rats were divided into 8 groups:control, morphine treated group (20 mg/kg/day); Resveratrol -treated groups (2, 8, 20 mg/kg/day);and morphine and Resveratrol treated group were administered interperitoneally for 14 consequent days. These mice were randomly assigned to 8 groups (n=6) and sperm parameters (sperm cell viability, count, motility and morphology), testis weight, testis histology and testosterone hormone and nitric oxide were analyzed and compared. Then the data were P<0.05 was considered significant. The results indicated that morphine administration significantly decreased testosterone level, count, viability and motility of sperm cells and testis weight and increase nitric oxidecompared to the saline group (P=000). However, administration of resveratrol and resveratrol plus morphine significantly boosted motility, count, viability of sperm cells, somniferous tubule diameter and testosterone while the decrease nitric oxide level in all groups compared to morphine group (P<0/025).It seems that resveratroladministration could increase the quality of spermatozoa and prevented morphine-induced adverse effects on sperm parameters.

Keywords:

Resveratrol , Sperm Parametrs , Morphine , Mice , Spermatozoa , Testosterone