Magnetic Nanoparticles Cytotoxicity Effects on Mouse GV Oocytes

Background: Recently, nanoparticles are widely used in diagnostic medicine or treatment. Vitrification of oocytes is a promising technique to preserve fertility, but it has deleterious effects on oocyte quality and leads to low-quality embryos. Supplementation of vitrification solution by nanoparticles could improve oocytes modality and its production competence in high quality embryos. Methods: To assess the toxicity effects of magnetic nanoparticles (Fe3O4), immature oocytes (GVs) were derived from 6-8 weeks-old NMRI mice and exposed to equilibration medium (7.5%EG plus 7.5%DMSO (v/v)) 5 min, then treated with vitrification solution (VS) (15%DMSO, 15%EG, 0.5 mol/L sucrose) supplemented by nanoparticles (VS+5: 5μgr/ml, VS+10: 10μgr/ml or VS+20: 20μgr/ml) 1 min, then they were exposed to warming solutions in three-step sucrose dilution: W1 (1.0 mol/L sucrose) 370c, W2 (0.5 mol/L sucrose) and W3 (0.25 mol/L sucrose) RT for 1, 3 and 3 min. IVM were performed for warmed immature oocytes.Viability and maturation rates were measured by trypan blue and hoechst staining. Results: IVM rate significantly decreased in VS+20 as compared to control and VS groups butthere were no analytically differences between VS and control groups in comparison with VS+5. Treatment of oocytes by VS+10 and VS+20 caused dramatic decrease in oocyte viability rate as compared to control group but this parameter didn’t show any comparative reduction in VS+5 versus control group (P<0.05). Conclusion: Using 5μgr/ml Fe3O4 nanoparticles in vitrification solution as compared to other concentration of nanoparticles did not have malicious effects on maturation and viability rates of mouse immature oocytes.

Keywords:

Oocyte , Vitrification , Nanoparticles , in Vitro Maturation