Three-Dimensional Wet-Electrospun Poly(Lactic Acid)/Multi-Wall Carbon Nanotubes Scaffold Induces Differentiation Of Human Menstrual Blood-Derived Stem Cells Into Germ-Like Cells

Infertility caused by the disruption or absence of germ cells is a major and largely incurable medical problem. Generation of gametes derived in vitro from stem cells hold promising prospects which could potentially help infertile men and women. Menstrual blood-derived stem cells are a unique stem cell source. To maintain the three dimensional structure of natural extra cellular matrices in vitro, scaffolds can do this favor and mimic a microenvironment for cell proliferation and differentiation. According to previous studies, poly (lactic acid) and multi-wall carbon nanotubes have been introduced as novel and promising biomaterials for the proliferation and differentiation of stem cells. This study designed a 3D wet-electrospun poly (lactic acid) and poly (lactic acid)/multi-wall carbon nanotubes composite scaffold to compare infiltration, proliferation, and differentiation potential of menstrual blood-derived stem cells toward germ cell lineage with 2D culture. Our primary data revealed that the fabricated scaffold has mechanical and biological suitable qualities for supporting and attachments of stem cells. The differentiated menstrual blood-derived stem cells tracking in scaffolds using scanning electron microscopy confirmed cell attachment, aggregation, and distribution on the porous scaffold. Based on the differentiation assay by RTPCR analysis, stem cells and germ-like cells markers were expressed in 3D groups as well as 2D one. It seems that poly (lactic acid)/multi-wall carbon nanotubes scaffold-seeded menstrual blood-derived stem cells could be viewed as a novel, safe, and accessible construct for these cells, as they enhance germ-like generation from menstrual blood-derived stem cells.

Keywords:

3D Scaffolds , Wet-Electrospun Poly (Lactic Acid)/Multi-Wall Carbon Nanotubes Nanofiber , Menstrual Blood Stem Cells , Germlike Cells , Infer Tility