Effects Of Methamphetamine on Spermatogenesis Indices Of Adult Male Rats

Background: Several studies have shown that Methamphetamine (MAMP) has inhibitory effects on oogenesis and spermatogenesis, and causes impair fertility. In this study, we investigated the effect of MAMP administration on permatogenesis indices in the testis of adult male rats. Methods: In this experimental study, 50 male Wistar rats were randomly divided into control (received no treatment) (n=10), Vehicle (received saline for 7 and 14 days) (n=20) and experimental (received MAMP (5 ml/kg, IP) for 7 and 14 days) (n=20). We count the number of spermatogonia, spermatocytes and Leydig cells. Spermatogenesis indices which include: tubular differentiation index (TDI), spermiogenesis index (SI), repopulation index (RI) and the mean seminiferous tubules diameter (MSTD), were studied. Data were analyzed by one-way ANOVA, using SPSS software version 20. Pvalue <0.05 was considered statistically significant. Results: This study showed that MAMP caused a significant decrease in number of seminiferous tubules cells and spermatogenesis in treated group compared with the control. Also, results showed a significant decrease in spermatogenesis indices including TDI, SI, RI and MSTD in 14th day, compared with control group (P<0.001).Conclusion: The data showed the adverse effects of methamphetamine administration (for 7 and 14 days) on testes structure and spermatogenesis indices in rat testis tissue. The underlying mechanism(s) needs further investigation

Keywords:

Methamphetamine , Spermatogenesis index , Testis , Rat