Antioxidative Effects of Cerium Dioxide Nanoparticles Ameliorate Diabetes -Induced Testicular Damages

Backgournd: CeO2 nanoparticles have been shown to act as an anti-oxidant and anti-inflammatory agent in the treatment of several diseases including cancer and diabetes. Oxidative stress may play an important role in the pathophysiology of diabetes-related male reproductive dysfunction and abnormalities. In the present study, we examined the effects of cerium oxide (CeO2) nanoparticles on sperm parameters and spermatogenesis, in diabetic rats.Methods : We included 24 Adult male Wistar rats weighing 250-300 g. After a week of adaptation to the standard diet, the rats were randomly divided into four groups with six rats in each. Control group received only a standard diet. CNPs group received CNPs 30 mg/kg/daily, diabetic rats received STZ (60mg/kg/daily), rats in STZ+ CNPs group received 30mg/kg/2 weeks of CNPs following STZ injection. We assessed sex hormones, sperm parameters and spermatogenesis, and Sperm DNA Fragmentation. Results: The almost all of the sperm parameters (count, motility, viability and normal morphology) in the diabetic rat was reversed by CeO2 nanoparticles administration. We showed that after CNPs administration, sperm DNA fragmentation significantly reduced in the STZ treated rats. Leydig cells, Sertoli cells, spermatids, primary spermatocytes and spermatogonia reduced significantly in the diabetic rats and CNPs treatment was effective in the most of the cases.Conclusions: Our study concluded the CeO2 nanoparticles can attenuate the detrimental effects of diabetes on the sperm potential fertility, sperm parameters, DNA integrity and testicular histology.

Keywords:

Diabetes , Ceo2 Nanoparticles , SpermParameters , Oxidative Stress