The Effects of Dietary Fatty Acids on The Expression of 17βhsd Isoforms Genes in Mouse Adipose Tissue and Sperm Parameters

Background: Adipose tissue (AT) constitutes an important site for steroid hormone metabolism, but little information exists on the effective use of dietary fatty acids on AT metabolism and gene expression. Our research objective was to investigate changes in the exprHVVLRQ RI 17 +6 isoforms genes in mice AT which consumed tallow (trans and saturated fatty acid source) in comparison to mice which fed with control diet. Methods: Mature male mice (8 weeks) were divided into fat diet (FD) (n=15) and control (n=14) groups. FD fed tallow for 2 months. Abdominal AT samples were collected and performed quantitative RT-PCR. Also, the parameters of sperm were measured by CASA. Data were analyzed by SPSS (version20) . Results :The sperm concentration (5.9 vs. 10.6 M/ml), total motility (29.3 vs. 61.5 %), and progressive motility (23 vs. 52 %) were significantly lower in FD than control (p <0.05).17 +6 12 DQG 17 +6 7 WUDQVFULSW OHYHOV significantly increased in FD than control (p <0.05). 7KH H[SUHVVLRQ RI 17 +6 5 DQG 17 +6 3 GLG Qot change significantly. Negative significant correlations were found in the 17 +6 12 (U -0.452) DQG 17 +6 7 (U -0.585) ZLWK VSHUP FRQFHQWUDWLRQ 17 +6 12 (U -0.404)DQG 17 +6 7 (U -0.454) with sperm motility (p<0.05).Conclusions: Significant increaseV RI 17 +6 12 DQG 17 +6 7 (HVWURJHQLF) QR FKDQJH LQ 17 +6 5 DQG 17 +6 3 (DQGURJHQLF) VKRZHG that dietary trans and saturated fatty acid have effects on AT gene expression via estrogenic pathways.

Keywords:

Mice , Adipose Tissue , 17βhsd Isoforms , Dietary Fatty Acids