Reperfusion Reverses the Torsion-Induced DNA Damage By Initiating DNA Repairmen in Experimental Modals; Evidence For PCNA Up-Regulation

Background: It has been shown that, the testicular torsion is one of the most important urological emergencies, which negatively impacts testicular tissue, in a duration and degree manner and consequently leads to complete infertility. Moreover, it has been reporeted that, reperfusion is the only emergency intervention to treat this syndrome. Therefore, the present tried to illustrate the effects of reperfusion on torsion-induced pathogenesis by evaluating testicular tissue damage, DNA fragmentation ratio and PCNA gene expression. Methods: To follow-up current study, 30 mature male Wistar rats (NO=6 rats in each group) were used. Following 4 hours from torsion induction,the reperfusion was induced. Then, the animals were subdivided into; 4 hours torsion-induced (T1), (b) 1hour post-reperfusion (T2), 2 hours post-reperfusion (T3), 4 hours post reperfusion (T4) and 8 hours post-reperfusion (T5) groups. The seminiferous tubules differentiation (TDI) and spermiogenesis (SPI) indices were investigated and compared between groups. The PCNA mRNA level was analyzed using Reverse Transcriptase-PCR (RT-PCR). Moreover, the PCNA+ cell numbers per mm2 of tissue wasassessed using immunohistochemical staining.Finally, the testicular DNA fragmentation was analyzed using DNA ladder test. Results: Observations demonstrated that, the reperfusion (albeit after 8 hours in T5 group) significantly (P<0.05) enhanced tubular TDI and SPI ratio and up-regulated the PCNA expressions versus T1 group. Moreover, the animals in T5 group showed diminished DNA fragmentation ratio versus T1 group. Conclusion: Minimum 8 hours, post reperfusion is needed to re-initiate necessary expressions of PCNA to restore cell cycling machinery and ameliorate torsion-induced DNA damage.

Keywords:

Torsion , Reperfusion , DNA Damage , PCNA , Rat