Extraction and biochemical characterization of a peroxidase from soybean (Glycine max) seed coats
Publish place: 06th International Congress on Chemical Engineering
Publish Year: 1388
نوع سند: مقاله کنفرانسی
زبان: English
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شناسه ملی سند علمی:
ICHEC06_390
تاریخ نمایه سازی: 1 مهر 1388
Abstract:
Plants food wastes have been given much less attention or even disregarded. In some instances, however, oxidative enzymes from residual plant tissues have been shown to effectively degrade recalcitrant pollutants. Soybean seed coat peroxidase (SBP) is an inexpensive oxidoreductive enzyme and could potentially be used to oxidise/polymerise various organic pollutants present in the industrial and petrochemical wastes. SBP is able to retain its catalytic properties under wide ranges of pH and temperatures. In this study, a systematic evaluation of the biocatalytic properties of SBP was carried out. The enzyme exhibited highest activity and stability at pH 6.0 and retained over 75% of the maximumactivity for 12 hours. SBP also showed 2.5 times higher activity at a temperature of 65°C when compared to the activity at room temperature. The SBP was found to be heat-stable with 95% of the enzymatic activity remaining after heat treatment at 75°C and for 30min. The pH and temperature of the reaction mixture were found to significantly influence the SBP activity.
Keywords:
catalytic properties , enzyme activity , organic solvents , oxidoreductive , soybean seed coat peroxidase
Authors
Farnoush Ghaemmaghami
Petroleum and Chemical engineering department, Sharif University of Technology P.O.Box ۱۱۳۶۵-۹۴۶۵, Tehran, Iran
Sepideh Motamed
Petroleum and Chemical engineering department, Sharif University of Technology P.O.Box ۱۱۳۶۵-۹۴۶۵, Tehran, Iran
Iran Alemzadeh
Petroleum and Chemical engineering department, Sharif University of Technology P.O.Box ۱۱۳۶۵-۹۴۶۵, Tehran, Iran
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