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Paper
Title

Optimization of Plant Peroxidase Purification

Year: 1388
COI: ICHEC06_392
Language: EnglishView: 1,496
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Authors

Sepideh Motamed - Department of Chemical & Petroleum Engineering, Sharif University of Technology, P.O. Box: ۱۱۱۵۵-۹۴۶۵, Azadi Ave., Tehran, Iran
Farnoosh Ghaemmaghami - Department of Chemical & Petroleum Engineering, Sharif University of Technology, P.O. Box: ۱۱۱۵۵-۹۴۶۵, Azadi Ave., Tehran, Iran
Iran Alemzadeh - Department of Chemical & Petroleum Engineering, Sharif University of Technology, P.O. Box: ۱۱۱۵۵-۹۴۶۵, Azadi Ave., Tehran, Iran

Abstract:

Partial purification for plant peroxidase from white radish was optimized. Precipitation by ammonium sulfate and aqueous two phase system (as two parallel methods) were used. Polyethylene glycol/ammonium sulphate/NaCl (27:6.0:2.0%, w/v) system followed by gel filtration chromatography gave purification factor of about 34 fold. On the other hand, ammonium sulfate precipitation (40-80% degree) followed by gel filtration gave only 16.5 purification fold. Hence, aqueous two phase system was more efficient and useful primary purification step since it was less laborious, less time consuming, and led to more purification factor. The partially purified white radish enzyme was stable at pH range 4.0-8.0 and had an optimum pH at 4.0. The enzyme was stable at 65°C with an optimum activity at 55°C.

Keywords:

Paper COI Code

This Paper COI Code is ICHEC06_392. Also You can use the following address to link to this article. This link is permanent and is used as an article registration confirmation in the Civilica reference:

https://civilica.com/doc/77665/

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Motamed, Sepideh and Ghaemmaghami, Farnoosh and Alemzadeh, Iran,1388,Optimization of Plant Peroxidase Purification,06th International Congress on Chemical Engineering,Hajjiabad,https://civilica.com/doc/77665

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