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Title

Optimization of alpha amylase production by solid-state fermentation of Bacillus sp.KR-8104 in a laboratory scale packed bed bioreactor

Year: 1388
COI: ICHEC06_407
Language: EnglishView: 1,644
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Authors

Sorour Derakhti - Biotechnology Group, Chemical Engineering Department, Faculty of Engineering, Tarbiat Modares University.
Seyed Abbas Shojaosadati - Biotechnology Group, Chemical Engineering Department, Faculty of Engineering, Tarbiat Modares University.
Khosro Khajeh - Biotechnology Group, Chemical Engineering Department, Faculty of Engineering, Tarbiat Modares University.

Abstract:

In this study the production of α-amylase by cultivation of Bacillus sp. KR-8104 on wheat bran under solid-state fermentation in a laboratory scale packed bed bioreactor was investigated. This strain is capable to produce a Ca independent α- amylase, which is active and stable at low pH values (4–6). The influence of airflow rate, initial substrate moisture and temperature on enzyme production was evaluated using response surface methodology based on Box-Behenken design. The experimental data was fitted to a polynomial model for the yield of enzyme, which revealedthe optimum levels of tested variables as follows: temperature 37 °C, initial moisture 72 % and airflow rate 0.15 L/min. The average α-amylase activity, 473.8 U/g dry fermented solids (in triplicate), under optimal conditions was obtained.

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Paper COI Code

This Paper COI Code is ICHEC06_407. Also You can use the following address to link to this article. This link is permanent and is used as an article registration confirmation in the Civilica reference:

https://civilica.com/doc/77680/

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Derakhti, Sorour and Shojaosadati, Seyed Abbas and Khajeh, Khosro,1388,Optimization of alpha amylase production by solid-state fermentation of Bacillus sp.KR-8104 in a laboratory scale packed bed bioreactor,06th International Congress on Chemical Engineering,Hajjiabad,https://civilica.com/doc/77680

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