Over-determined metabolic network model for metabolic flux analysis of mycophenolic acid production by Penicillium bervicompactum

In this study, metabolic flux analysis method was applied for calculation of metabolic flux distribution of mycophenolic acid (MPA) biosynthesis by Penicillium bervicompactum. An over-determined metabolic network model including 68 biochemical reactions and 70 cell metabolites was developed for MPA biosynthesis in P. bervicompactum. Three extracellular fluxes (glucose consumption and biomass and MPA production fluxes) in steady-state condition in a continuous 2-L stirred tank bioreactor were determined. Stoichiometric representations of 68 metabolic reactions, mass balance equations for all cell metabolites and a 70×68 stoichiometric coefficients matrix were determined as tools for analysis of the metabolic fluxes. Metabolic flux distribution was calculated by least-square method using MatLab software. Finally, consistency of the developed metabolic model was approved with calculated extracellular metabolites fluxes

Keywords:

Metabolic flux analysis , Mycophenolic acid , Penicillium bervicompactum , Metabolic network model