DETECTION OF MECA GENE AMONG METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS ISOLATED FROM BLOOD INFECTION

Background and Aim:methicillin resistant staphylococcus aureus is one of the main agents rising significantly in both hospital and community acquired infections. The aim of this study was to analyze the prevalence of the MRSA among Bloodstream infection in Qom hospitals and determinative the antibiotic resistance of the isolates.Methods:During three years (2013-2015) S.aureus was isolated from bloodstream infected patients at Qom Hospitals and their antibiotic susceptibility pattern to different antibiotics were determined. Finally, using PCR, the methicillin resistant isolates were tested for the presence of mecA gene.Results:In this study, a total of 500 blood samples, 140 (28%) isolates were S.aureus, 48 (34.28%) were found resistant to cefoxitin disk, according to CLSI and mecA gene was detected in 48 strains (34.28%). Our study results showed that the agar screen technique like PCR is a reliable in determining of MRSA strains.Conclusion:In this study, we found high prevalence of MRSA and the mecA is widespread in S. aureus strains isolated at Qom hospital.

Keywords:

Bloodstreem infection , S.aureus , MRSA , mecA