IDENTIFICATION OF KPC BETA-LACTAMASE GENE IN KLEBSIELLA PNEUMONIA ISOLATES ISOLATED FROM PULMONARY SECRETIONS

Background and Aim: Klebsiella pneumoniae is an important human pathogen which has been considered due to the development of resistant infections in hospitals. The Multiple drug resistance, especially in urinary tract infections, has led to development of several problems. The aim of this study was to determine the prevalence of KPC β-lactamase in Klebsiella pneumonia isolates isolated from patients with pulmonary infections in Qom.Methods: This cross-sectional study was performed on 200 pulmonary samples obtained from hospitalized patients and outpatients during a one-year period. Diagnosis was done using biochemical methods. Antibiotic susceptibility was evaluated by disc diffusion method based on CLSI2013 criteria. PCR was used for identification and amplification of blaKPC genes in Klebsiella pneumoniae isolates.Results:Among 200 cultured pulmonary samples, 30 samples of Klebsiella pneumoniae were isolated. Isolates were resistant to more than three classes of antibiotics. The results of antibiotic resistance analysis of isolates showed that the highest resistance was obtained against trimethoprim with a resistance of 47.98%, cefalotin with 59.53% and nalidixic acid with 79.43%; however, the highest sensitivity was obtained against amikacin with 90.95% and meropenem with 37.83% and imipenemt with 62.81% respectively. The results of PCR showed that among 30 Klebsiella pneumonia, 10 isolates (33.33%) contained blaKPC gene.Conclusion:The blaKPC enzyme is one of the most common β-lactamase enzymes. Optimization of the use of anti-microbial agents in order to control the infection and also in order to prevent increase in the population of two or three antibiotics resistant bacteria is strongly important.

Keywords:

KPC beta-lactamase gene , Klebsiella pneumonia , pulmonary secretions