EFFECTS OF METHANOLIC EXTRACT OF CALCEOLARIA HERBEOHYBRIDA ON MICROORGANISMS

Background and Aim:From the past to the present, plants have been used in the treatment of diseases. One of these diseases is infectious diseases. For this purpose, plant extracts are used. Different solvents have been used for the preparation of plant extraction. In this research study on antimicrobial effects of ornamental plant namely Calceolaria herbeohybrida.Methods:The plant material used in this study was the flower of Calceolaria herbeohybrida. The flower, stem, and leaf of the plant were harvested and identified at Kharazmi University, Farabi Herbarium. 10 gram of fresh flowers were dried, grained and macerated in 80% methanol, overnight. The macerates were then concentrated with solvent evaporation and then 1 ml of sterile distilled water was added to solve it. The Antimicrobial activity of this concentrated extract, performed with well-diffusion agar method on E.coli، P.aeroginosa, MRSA, B.subtilis and, C.albicans.Results:According to other research paper, strains showing an inhibition zone more than 1 mm were considered as sensitive strains. So all of the used strains in this research were resistant to methanol extraction of Calceolaria herbeohybrida.Conclusion:There was no effective constitute in methanolic extract of Calceolaria herbeohybrida as the antimicrobial agent against used strains. So this plant cannot use for treatment of infectious disease.

Keywords:

Calceolaria herbeohybrida , diffusion , methanol , extraction