ANTIBIOFILM POTENTIAL OF CURCUMIN AGAINST AEROMONAS HYDROPHILA FISH ISOLATES

Background and Aim:Aeromonas hydrophila is a Gram-negative bacterium that can cause a variety of infections in fish and humans. Curcumin is a natural component of Curcuma longa rhizome. Several studies have reported the broad spectrum biological activities of curcumin such as anti-inflammatory, immunomodulatory, antioxidant, anticancer and antimicrobial effects. Biofilm formation is regarded as the main determinant factor in establishment of infection and bacterial pathogenicity. Antibiofilm potential of Curcumin has rarely been investigated. Thus, in this study antibiofilm activity of Curcumin was investigated.Methods:Briefly, cultures of A. hydrophila fish isolates with the approximate cell populations of 1.5×108 CFU/mL were incubated for 48 h at 28 ℃ in Mueller-Hinton broth containing different concentration of Curcumin (32-512µg/mL) in 96 well microtiter plates. Cultures were removed, biofilm was washed three times with distilled water and stained using 150 µl of 0.3% (w/v) crystal violet for 15 min at room temperature. Crystal violet was dissolved using 30% (v/v) glacial acetic acid for 20 min and the absorbance was measured at 550 nm.Results:Curcumin inhibited biofilm formation of A. hydrophila in a range of 31-75% compared to the control. In addition, the Biofilm inhibitory concentration (BIC) value of Curcumin was determined 64µg/mL.Conclusion:Curcumin efficiently inhibited biofilm formation of A. hydrophila and thus, could be regarded as an efficient antibiofilm agent which could be used for preventive and therapeutic approaches.

Keywords:

Curcumin , biofilm. Aeromonas , infection