THE DANGER OF INCREASE MBLS PRODUCTER IN HOSPITAL SAMPLES

Background and Aim: According to a report from the World Health Organization Acinetobacter baumannii and Pseudomonas aeruginosa are one of the most important pathogens causing disease in humans ,which also are one of the most important causes of nosocomiale in hospitalized patients. The purpose of this study was determine the VIM-1 gene in Acinetobacter baumannii and Pseudomonas aeruginosa isolated from clinical samples of Baqiyatallah HospitalMethods:In this study , 150 samples of Acinetobacter baumannii and Pseudomonas aeruginosa were collected from different clinical specimens of Baqiyatallah Hospital during years 2016-2017 . First , collected isolates were confirmed by biochemical methods and then were detected by disc diffusion method according to CLSI 2017 for several antibiotics. Combined disc methods were used to determine the phenotypes of MBLs producing isolates and at the end , study presence of the VIM-1 gene by PCR methods.Results:The most antibiotic resistance in Acinetobacter baumannii and Pseudomonas aeruginosa was related to cefotaxime(100%) and ciprofloxacin (94.93%), respectively. Also ,using the combined disc method show that 78.83% and 87.64% of Acinetobacter baumannii and Pseudomonas aeruginosa isolates are the producer of MBLs. Molecular studies also shows that the prevalence of VIM gene in these Acinetobacter baumannii and Pseudomonas aeruginosa isolates was 64.38% and 100% , respectively.Conclusion:This study demonstrates increase antibiotic resistance in both Acinetobacter baumani and Pseudomonas aeruginisa isolate . Control of the use types of antibiotics and the replacement of a more suitable treatment than antibiotic for treatment of infection are best solve to prevent the harms caused of increase resistance antibiotic.

Keywords:

MBL.Pseudomonas aeruginosa.Acinetobacter baumannii