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: DETERMINATION OF ANTIBIOTIC RESISTANCE PATTERN AND THE PREVALENCE OF BETA-LACTAMASE GENE BLATEM, SHV AND DHA IN PSEUDOMONAS AERUGINOSA CLINICAL SAMPLES

عنوان مقاله: : DETERMINATION OF ANTIBIOTIC RESISTANCE PATTERN AND THE PREVALENCE OF BETA-LACTAMASE GENE BLATEM, SHV AND DHA IN PSEUDOMONAS AERUGINOSA CLINICAL SAMPLES
شناسه (COI) مقاله: MEDISM19_195
منتشر شده در نوزدهمین کنگره بین المللی میکروب شناسی ایران در سال 1397
مشخصات نویسندگان مقاله:

Reza Yari - Assistant Professor of Cell Biology, Department of Biology, Faculty of Sciences, Borujerd Branch, Iran
Mohammad Torkashvand - MSc. Student, Department of Biology, Faculty of sciences, Borujerd Branch, Islamic Azad University, Borujerd, Iran
Mohammad Reza Mehrabi - Department of Laboratory Sciences, Medical Faculty, Borujerd Branch, Islamic Azad University, Borujerd, Iran

خلاصه مقاله:
Background and Aim:The presence of ESBL genes play an important role in resistance to beta-lactam antibiotics producing strains of these enzymes play . Gram-negative bacteria including Pseudomonas aeruginosa resistance to various antibiotics , beta-lactams and carbapenems especially increasingly been reported . This study aimed to investigate the prevalence of beta-lactamase genes in Pseudomonas aeruginosa isolates was performed by PCR method.Methods:103 strains of Pseudomonas aeruginosa isolated from clinical samples of urine, sputum, burn wounds and devices dialysis pediatric hospitals in the cities of Qom and Tehran, with the Gram stain, a test of biochemical differentiation and PCR District 16s rRNA detection were identified. Resistance to Ceftazidime/clavulanic acid and cefotaxime/ clavulanic acid 10 other common antibiotics was determined by disk diffusion method. Genomic DNA was extracted by method kit was performed and the beta-lactamase gene amplification with primers Amplicon available and were designed and genes were analyzed by PCR.Results:Of the total 103 isolates of Pseudomonas aeruginosa, 50 isolates with broad-spectrum beta-lactamase phenotype were observed. 46 isolates (47/38%) were carriers of TEM-1 beta-lactamase gene. 35 isolates had SHV gene and none of the isolates was observed in DHA gene.Conclusion:Pseudomonas aeruginosa beta-lactamase largely resistant to other common antibiotics and to select a suitable drug for destroying their antibiotic susceptibility testing should be done.

کلمات کلیدی:
Pseudomonas aeruginosa, ESBL, blaTEM, blaSHV, blaDHA

صفحه اختصاصی مقاله و دریافت فایل کامل: https://civilica.com/doc/782534/