PHENOTYPIC AND GENOTYPIC DETECTION OF EXTENDED-SPECTRUM Β-LACTAMASE (ESBL)-PRODUCING ESCHERICHIA COLI IN PATIENTS WITH COMMUNITY-ACQUIRED URINARY TRACT INFECTIONS
Publish place: 19th International Congress of Microbiology of Iran
Publish Year: 1397
نوع سند: مقاله کنفرانسی
زبان: English
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شناسه ملی سند علمی:
MEDISM19_219
تاریخ نمایه سازی: 13 مهر 1397
Abstract:
Background and Aim:Extended-Spectrum β-lactamases (ESBLs) are enzymes which cause resistance to most cephalosporins, penicillins and monobactams. There were various groups of ESBLs, including CTX-M, SHV and TEM enzyme types. β-lactam antibiotics are widely used for treatment of urinary tract infections (UTIs). The presences of ESBL-producing E. coli in UTIs can lead to treatment failure with the listed classes of antibiotics. Therefore phenotypic or genotypic detection of these isolates is important.Methods:Seventy eight E. coli isolates were obtained from 78 urine samples of patients with community-acquired UTIs. The isolates were studied for ESBLs production by combination disk diffusion method using cefotaxime (30μg), ceftazidime (30μg), ceftriaxone (30μg) and cefotaxime-clavulanic acid (30/10μg) disks. Presence of blaTEM, blaSHV, blaCTX_M genes were determined by PCR.Results:Prevalence of resistance to ceftazidime, ceftriaxone and cefotaxime were 42.3%, 53.8% and 55.12%, respectively. Since the ≥5 mm increase in the zone of inhibition diameter for cefotaxime-clavulanic acid compared to zone for cefotaxime alone indicates ESBL activity, the prevalence of ESBL-producing E. coli was 41%. Among the 32 ESBLs- producing E. coli, 29(90.6%) harbored CTX-M, 19(59.3%) harbored SHV, 4(12.5%) harbored TEM, 1(3.1%) harbored both TEM and CTX-M, 17(53.1%) harbored both SHV and CTX-M, and 2(6.25%) harbored all the three types of ESBLs.Conclusion:The worldwide increase in prevalence and spread of ESBL- producing E. coli can limit therapeutic options for UTIs and addition of healthcare costs. Therefore, the proper usage of extended-spectrum cephalosporins, regular surveillance of antibiotic resistance of clinical isolates and detection of ESBL-producers by accurate laboratory testing methods are crucial.
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Authors
Zahra Naziri
Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
Abdollah Derakhshandeh
Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
Mohammad Motamedifar
Department of Bacteriology and Virology, Shiraz University of Medical Science, Shiraz, Iran
Meisam Poormaleknia
Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran