EVALUATING THE IN VITRO INHIBITORY EFFECT OF NANOEIZED PARTICLES OF PYRANS ON ESBLS PRODUCING ESCHERICHIA COLI STRAINS ISOLATED FROM CLINICAL SPECIMENS IN KARAJ CITY

Background and Aim:The increase of ESBLs producing Escherichia coli among patients with urinary tract infection is a significant concern. Drug resistance of these bacteria is very important especially in nosocomial infections. Because of acquiring plasmids encoding broad beta-lactamases, Escherichia coli has become resistant to beta-lactam antibiotics. Increasing in antibiotic resistance rushed researchers to the use of nanoparticles for creating modern remedies.Methods:This descriptive-observational study performed on 60 samples of Escherichia coli collected from Karaj city. Isolates were identified by biochemical standard tests. Antimicrobial susceptibility performed by four antibiotics, including Cefepime, Ceftazidime, Ceftriaxone and Cefotaxime. ESBLs producing isolates were screened by phenotypic confirmation test. Minimum inhibitory concentration (MIC) of pyran nanoparticles was determined by using sterile 96 microwell plates microbroth dilution method. The dilution range tested by using dimethyl sulfoxide, as solvent, was between 2.44-1250 mg/ml.Results:In total, 14 out of 60 Escherichia coli isolates have been observed as ESBLs producers. Antibiotic resistance level among isolates was as follows: FEB 23.33%, CAZ 16.66%, CRO 30% and CTX 31.66%. ESBL producing isolates were tested for MIC values. Bacterial growth wasn’t observed at 833.33 mg/ml of concentration which was considered as MIC. Furthermore MIC was equal to MBC.Conclusion:As a result, due to the ever-increasing number of antibiotic-resistant strains use of nanoparticles can help antimicrobials design and it can be a new hope for modern therapies design against bacterial threats and dilemmas.

Keywords:

ESBL genetic Escherichia coli , Drug resistance , Nano-particles