EVALUATION OF SEVERAL PHENOTYPIC METHODS FOR DETECTION OF KPC AND MBL PRODUCTION IN PSEUDOMONAS AERUGINOSA ISOLATES

Background and Aim:Carbapenems are one of the first-choice antibiotics for the treatment of Pseudomonas aeruginosa infections. Therefore, early and correct identification of carbapenemase-producing isolates for management of antimicrobial therapy is very important.Methods: A total 97 of Pseudomonas aeruginosa isolates from different clinical samples were collected during the September 2017 to February 2018. Antibiotic susceptibility tests were conducted by Kirby-Bauer disc diffusion according to the clinical and laboratory standards institute (CLSI) guidelines. Determination the minimum inhibitory concentration was done by E-test. To identify metallo-betalactamase enzymes, combined disk tests (CDT) carbapenem inactivation method (CIM) and Modified Hodge test (MHT) were used.Results:From 97 P. aeruginosa isolates, 47 (48/4 %) were carbapenem resistant. Among these, 27 (57/44%) were identified as MBL-producing P.aeruginosa isolates using DDST, 14 (29/78%) CIM positive and 25 (53/19%) KPC were positive.Conclusion:Our results showed that combined disk tests (CDT), carbapenem inactivation method (CIM) and Modified Hodge test are very sensitive and useful tools which can be performed in the routine laboratory test for identification of carbapenemase producing in P. aeruginosa isolates.

Keywords:

KPC , MBL , Pseudomonas aeruginosa