Shahrzad Soumeh Saraei Sabet - life science department, islamic azad university of gorgan, gorgan, iran
Teena Dadgar - life science department, islamic azad university of gorgan, gorgan, iran
Hadi Bazzazi - life science department, islamic azad university of gorgan, gorgan, iran

Background and Aim:World health organization has published its first ever list of antibiotic-resistant priority pathogens – a catalog of 12 families of bacteria that pose the greatest threat to human health. In this list, Pseudomonas aeruginosa and Staphylococcus aureus placed in priority one and priority two respectively. The aim of this study was the investigation of the antibacterial effect of amniotic membrane and PRP on beta-lactamase producing Pseudomonas aeruginosa and Staphylococcus aureus to find a natural source of antibacterial agents against drug-resistance pathogens.Methods:PRP was ordered from Iranian blood transfusion organization, and the amniotic membranes were collected aseptically from Gorgan city s hospitals from pregnant mothers at parturition day. Beta-lactamase producer Pseudomonas aeruginosa and Staphylococcus aureus isolates identification were performed with microbiological methods, iodometric test, and CLSIA protocol. Then isolates were cultured on MHA medium, and bactericidal effects of amniotic membrane and PRP were assessed using well diffusion and disk diffusion method respectively.Results:Results showed that among all of the isolates, six isolates of Pseudomonas aeruginosa and six isolates of Staphylococcus aureus were beta-lactamase producer. PRP has an antibacterial effect on all of six MRSA isolates while does not affect any of six Pseudomonas aeruginosa isolates. Also, results inferred that amniotic membrane has an antibacterial effect on all of the both MRSA and Pseudomonas aeruginosa isolatesConclusion:Amniotic membrane and PRP showed strong antibacterial potential against highly dangerous multi-drug resistance pathogens. Therefore human source natural products must be considered as a new source of antibacterial for drug discovery to address concerns about multi-drug resistance pathogens.

Pseudomonas aeruginosa, Staphylococcus aureus, Multi dug-resistance, Amniotic membrane, platelet concentrate