Behnaz Asadi - Department of Biology, Yadegar-e-Imam Khomeini (RAH) Shahre Rey Branch, Islamic Azad University, Tehran, Iran
Shiva Khalil-Moghaddam - Department of Biology, Yadegar-e-Imam Khomeini (RAH) Shahre Rey Branch, Islamic Azad University, Tehran, Iran
Atousa Aliahmadi - Medicinal Plants and Drug Research Institute, Shahid Beheshti University, Tehran, Iran

Background and Aim:In response to the propagation of bacteria resistant to many antibiotics also called multi-drug resistant bacteria, the discovery of new resources for producing agents that could act as alternatives to antibiotics is primordial. The aim of this study was to evaluate the antibacterial activity of Cruciata Tauriaca extracts against two Gram-posetive bacterial species..Methods:This plant have been collected in spring. Methanol and n-hexan was used for extraction of plant polar and nonpolar materials. Then minimum inhibitory concentration (MIC) of the plant materials were determined against Bacillus cereus PTCC 1015 and Staphylococcus aureus ATCC 25923 by broth micro-dilution method. Mueller hinton broth was used for preparation of serial diluted samples which were checked against 0.5-1 × 106 CFU (Colony forming unit) of bacteria. Incubation was done for 20 hrs at 37°C. MICs were recorded as the lowest concentrations which could inhibit visible growth of bacterium. Chloramphenicol was used as standard antibiotic.Results:Methanolic extract of Cruciata Tauriaca could show the best result (MICs 0.467 mg/ml for Bacillus cereus) and Staphylococcus aureus was inhibited in higher concentration of methanolic extract. (3.74 mg/ml) and hexane extract could show the best result for Staphylococcus aureus(MICs 0.116 mg/ml).Conclusion:According to the result of this study, methanolic and hexane extracts can exhibit antibacterial effects.

Antibacterial activity, Cruciata Tauriaca