Mohammad Motamedifar - Department of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences Shiraz, Iran
Yalda Malekzadegan - Department of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences Shiraz, Iran
Ali Abdi - Department of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences Shiraz, Iran
Ali Mirzaei - Student Research Committee, Shiraz University of Medical Sciences Shiraz, Iran

Background and Aim:Corneal ulcer is one of the ophthalmology emergencies that rapid diagnosis and treatment is important in preventing side effects. Two important infectious agents of corneal ulcer are Staphylococci spp. and Pseudomonas isolated from corneal ulcer to current antibiotics.Methods:The study is performed on 60 corneal ulcer samples. After diagnosing corneal infectious ulcers and taking samples from the active site of the wound, the specimens were transferred and incubated, and faced samples which pseudomonas was isolated to 11 different antibiotics and faced isolated staphylococcus to 9 different antibiotic.Results:From 60 samples collected, 7 pseudomonas, 3 Methicillin Sensitive Staphylococcus aureus (MSSA), 2 Methicillin Sensitive Coagulase Negative Staphylococcus aureus (MSCONS), 1 Methicillin Resistance Coagulase Negative Staphylococcus (MRCONS) and 1 Methicillin Resistance Staphylococcus aureus were isolated. Pseudomonas had the highest susceptibility to amikacin, colistin and levofloxacin, and staphylococci was 100% sensitive to amikacin and rifampin.Conclusion:In an experimental treatment of corneal ulcer, use of an aminoglycoside along with fluoroquinolone is recommended for complete coverage of Staphylococcus and Pseudomonas.

Corneal ulcer, Pseudomonas aeroginosa, Staphylococcus spp