SILVER NANOPARTICLES ECOFRIENDLY SYNTHESIS BY SATUREJA HORTENSIS AND EVALUATION ANTIBACTERIAL PROPERTIES
Publish place: 19th International Congress of Microbiology of Iran
Publish Year: 1397
نوع سند: مقاله کنفرانسی
زبان: English
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شناسه ملی سند علمی:
MEDISM19_256
تاریخ نمایه سازی: 13 مهر 1397
Abstract:
Background and Aim:Ecofriendly or Green synthesis of metal nanoparticles has become an important branch of nanotechnology and there is an increasing commercial demand for nanoparticles due to their wide applications.The aim of this work is to green synthesis of silver nanoparticles and to evaluate its Review of the properties of nanoparticles and antibacterial activity.Methods:In this study AgNPs were prepared by the reaction of 1mM silver nitrate and extracts of Satureja hortensis lesf , the reaction did at room temperature and the color changed from pale yellow to dark showed the silver nanoparticles were generated . Antibacterial activity of AgNPs was assessed by using disc diffusion method against Staphylococcus aureus PTCC 1431, Bacillus cereus 1015 PTCC and Escherichia coli PTCC 1399, Pseudomonas aeruginosa PTCC 1571 and Acinetobacter baumannii PTCC 1855 . The AgNPs were characterized by UV-visible spectrophotometer,particle size analyzer by dynamic light scattering method,and scanning electron microscopy techniques.Results:The formation of silver nanoparticles was confirmed by the presence of an absorption peak at 450 nm using spectrophotometer. The shape of particles was spherical and average size of them was about 73 nm. Antibacterial activity was observed against all tested bacteria,so that most antibacterial effect observed on P.aeroginosa and E.coli,on the other hand the lowest effect was observed on B.cereus and A.baumannii .Conclusion:results showed biological AgNPs and extract of Satureja hortensis leaf as well as AgNO3 have antimicrobial properties against pathogenic bacteria,but the most antimicrobial effects were observed by Ag NPs.
Keywords:
Antibacterial .Ecofriendly Synthesis . Nanoparticle .Satureja hortensis . Silver
Authors
Behnam Rafiee
PhD student of Bacteriology ,Department of Pathobiology, Faculty of Veterinary Medicine, Shahrekord University, shahrkord .Iran
Sepideh Ghani
PhD student of Medical Biotechnology , Shahid Beheshti University of Medical Science, Tehran,iran
Amin Nematolahi
Associate Professor Food Hygiene and Quality Control Faculty of Veterinary Medicine. Shahrekord University, Iran
Davoud Sadeghi
Biology Research Center, Faculty of Science & Basic Sciences, Imam Hossein University of Technology, Tehran, I.R.Iran