MOLECULAR AND SEROLOGICAL DETECTION OF MYCOPLASMA PNEUMONIAE AND DETERMINE OF THE CIPROFLOXACIN RESISTANCE PATTERN

Background and Aim:M. pneumoniae, which causes atypical pneumonia, is a well established pathogen of the respiratory tract. This Bacteria is intrinsically susceptible to fluoroquinolones. But Recently, drug-resistant forms of this bacteria have been reported. aim of this study is determine of prevalence of this bacateria by ELISA and PCR and MIC to ciprofloxacinMethods:The clinical samples (blood and nasopharyngeal swab) were collected from 135 patients who was referred to selective hospitals in Tehran with respiratory complaints were enrolled in 2017. nasopharyngeal swab sample collections were cultured on PPLO broth and PPLO agar. After culturing and DNA extraction with phenol / chloroform, polymerase chain reaction was performed by specific P1 gene’s primers. Ciprofloxacin’s MIC of Mycoplasma pneumonia Isolated was determined by Micro-broth dilution method . Also measure serum IgG antibody titers were measured by ELISA Mycoplasma pneumonia.Results:In this study, out of 135 samples 32, bacteria were isolated on PPLO agar. Using specific primers, 24 samples (17%) of Maycoplasma speciesiem- specific were positive for the presence of Mycoplasma pneumoniae. 15 Ciprofloxacin resistant isolates were evaluated. 35/5% of patients had positive antibody and 2/2% had borderline antibody and in 62/3% antibody titer was negative.Conclusion:This study showed that PCR is a sensitive and reliable method for rapid detection of Mycoplasma pneumoniae bacteria in respiratory infectious samples. but the results of this method are different from the ELISA method. also, It seams that the Resistance to ciprofloxacin is Relative common among Mycoplasma pneumoniae.

Keywords:

ciprofloxacin , Micro-broth dilution , Mycoplasma pneumoniae