OPTIMIZATION OF TRICHODERMA CULTURE MEDIUM USING DIFFERENT CARBON SOURCES TO INCREASE XYLANASE ENZYME PRODUCTION

Background and Aim:Trichoderma is one of ascomycete mesophile fungi, which exist in all soils and are part of the majority of soil’s cultivable fungi. Xylanases are part of hydrolases and possess single chains of glycoproteins whose weighs range from 6 to 8 KD. In this study, we try to. optimization of Trichoderma culture medium using different carbon sources to increase Xylanase enzyme production.Methods:We provided the standard Trichoderma reesei strain from the microbe collection of Iranian Research Organization for Science and Technology.Then,we measured the activity of its Xylanase enzyme using the standard method of DNSA. Next, for the sake of optimization, different parameters of the medium like carbon, examined.Results:The parental strain of Trichoderma reesei is featured by the xylanase enzyme activity of 2/59 U. the strain at the present of wheat cabran, respectively as the carbon, and at 7/5 pH and with the average of 35 °C had the most amount of production.Conclusion: Xylanase enzyme is one of the most important enzymes used in diverse industries but unfortunately is not produced in our country. As it can be seen from the results of this study, we can optimize the conditions for cultivating different organisms so as to increase the industrial production of various enzymes which are not produced in Iran. We hope, thereby, to make such a production easier at the industrial scale.

Keywords:

Trichoderma reesei , Xylanase , medium optimization Different sources of carbon