ISOLATION OF STREPTOMYCES BY ANTIDERMATOPHYTIC EFFECTS FROM SHORGHALE SOIL

Background and Aim:Todays drug resistance are increasing. Researchers are looking for alternative to replace common drug. One of this alternatives are Streptomyces. Streptomyces are filamentous bacteria that includes more than 500 species that isolation from soil and water. Some species of them can secretion antibiotics or other metabolites that kill or inhibit the growth of other microorganism. In this study we were screening for Streptomyces that had antidermatophyte effect on Microsporum canis from shorghale soil.Methods:For isolation of Streptomyces sample soil were tested by serial dilution method and culture in starch casein agar then incubated in 280c for 7 day. Gypsum and powder colonies were detected. For metabolite produced isolated bacteria were cultured in ISP2 broth in 28 0c and 150 rpm for 7 day. Then centrifuged and the same volume, ethyl acetate was added and incubated in 280c for 5h. Then liquid transfered to Separatory funnel. The supernatant phase was transferred to distiller. This liquid were used for anti dermatophyte effect. Microsporum canis was cultured in SCC agar. Antifungal effects studied by disk diffusion method and was compared with terbinafine and itraconazole.Results:Sixty Streptomyces were isolated from shorghle soil. One of them has shown a very good antifungal effect. The diameter of the non-growth zone was 29±1.24 , Results from 16srRNA indicate that this bacteria is 99.9% similar to Streptomyces albidochoromogenes.Conclusion:Streptomyces have high ability to produce antimicrobial metabolites. By purification and formulation these metabolites, they can be used of them as an alternative to common antibiotics or antiagents.

Keywords:

Streptomyces , dermatophytic , soil