ISOLATION AND IDENTIFICATION OF HEAVY METALS RESISTANCE BACTERIA FROM PARDIS MOUNTAIN IN JAM AREA AND STUDY OF LEAD BIOREMEDIATION BY THEM

Background and Aim:Pb (Lead) is one of the heavy metals. Heavy metals resistance in micro-organisms result from their exposure with these compounds. Environmental contaminations of soil, water and air by heavy metals are mainly produced by industrial activities. Human exposure with lead is very dangerous and it’s toxicity depends on the dosage and the route of exposure.Methods:Bioremediation by heavy metals resistance micro-organisms is the best technique that is very simple, fast and inexpensive. In this research 21heavy metals tolerant micro-organisms were isolated from Fajr Jam Gas Refinary Company (F.J.G.C) wastewater and water of subterranean canal and soil of Pardis mountain in Jam area that they can remediate high perecent of lead.Results:4strains of these micro-organisms were selected for molecular identification by 16 Sr DNA sequencing. The results showed that the strains (KP2A1 and KP4B2) isolated from Pardis mountain were (Pseudomonas stutzeri ATCC 17588(T) ) and (Pseudomonas songnenensis NEAU-ST5-5(T) ) respectively. KP2A1 could tolerant to lead nitrate up to 15mM concentration and had the ability to removed 72.8% of lead nitrate and KP4B2 could tolerant to lead nitrate up to 13mM concentration and removed 56.7% of lead nitrate. 2striains (LG3B1 and LG52B) that isolated from wastewater of (F.J.G.C) determined as (Bacillus paramycoides NH24A2(T) ) by molecular identification. This strain could tolerant lead up to 15mM concentration and they removed 67.2% and 65.1% of lead nitrate respectively.Conclusion:It is recommended to perform these experiments at a wider scale and use consortium of these 21 strains for lead bioremediation.

Keywords:

bioremediation , Heavy metals , lead , Pseudomonas stutzeri , Pseudomonas songnenensis , Bacillus paramycoides