Saeedeh Morovati - M.Sc of Microbiology, Islamic Azad University, Qom Branch, Qom, Iran
Mohammad Reza Zolfaghari - Associate professor of Micribiology Group, Islamic Azad University, Qom Branch, Qom, Iran
Behin Omidi - Assistant professor of Biology Group, Islamic Azad University, Central Branch, Tehran, Iran

Background and Aim:In recent years zinc oxide nanoparticles (ZnO-NPs) as an important ceramic materials. Its used in skin conditions, textile and automotive industries, and have anticancer, antibacterial propertiest. Zinc oxide nanoparticles was synthesized by physical and chemical methods but this method have been toxic effect The another method is Green synthesis that used plants, and microorganisms. Some microorganisms was used but the most of them had pathogenic effects. In this study we used Lactobacillus acidophillus as a probiotic to biosynthesis of ZnO-NpMethods:: Lactobacillus acidophillus (PTCC1643) was cultured in MRS broth and incubated in 37 ̊c and 5%co2 for 24 h Then its centrifuged in 4000 rpm for 20 min. Supernatant was filtered (by 0.45 micron) then supernatant was added to zinc nitrate 0.001M and incubated in 37c for 1 week. was used uv-vis spectrophotometer, XRD, XRF, AAA and TEMResults:Result was shown that Lactobacillus acidophillus could biosynthezed ZnONp. Strong Peak was observed at 374nm in UV-Vis spectrophotometer, XRD and XRF results shows that ZnO-NPs has been produced. Size of This ZnO-Nps was 45±0.12nm and their concentration was 150.75ppm.Conclusion:According to results, we have successfully extracellular synthesized of ZnO-NPs by Lactobacillus acidophillus supernatants. This way is simple, safe, low cost, effective and ecofriendly. In other hand Lactobacilluses are the major group of probiotics, then this bacteria are safe and without pathogenic effects. So we can used of them in medical and pharmacology purposed.

Lactobacillus acidophilus- Biosynthesis- Zinc oxide nanoparticles