ISOLATION, IDENTIFICATION AND OPTIMIZATION OF MICROBIAL OIL PRODUCTION AND EVALUATION OF THE ANTIBACTERIAL EFFECT

Background and Aim:Oleaginous yeasts have attracted much attention because of being single cells, lack of endotoxin and the possibility of large-scale biotechnological production. Oleaginous yeasts that are classified as GRAS (safety microorganisms) are suitable for single cell oil productionMethods:Evaluating of fatty acid profiles before and after optimization process using gas chromatography-mass spectrometry and finally evaluation of its antibacterial properties, were conducted. The best yeast strain was identified as Cryptococcus heimaeyensis using PCR method. After optimization of lipid production with Taguchi method, lipid production and lipid yield were reached to 8.05 g/L and 58.33%, respectivel. Esterified lipid composition contains linoleic acid, oleic acid and palmitic acid, as the most fatty acids in the fatty acid profile of the yeast. Oil extractions from yeasts have very similar fatty acid profile to vegetable oils and showed antimicrobial effect.Results: Isolation, identification and optimization of microbial oil production and evaluation of the antibacterial effect was studied in this studyConclusion:: Finding of native strains with high potential of lipid production and optimization through experimental design method is of great importance in the fields of biotechnological productions

Keywords:

microbial lipid , anti-bacterial effect , gas chromatography-mass spectrometry