OPTIMIZATION OF CARBON AND NITROGEN SOURCES FOR THE PRODUCTION OF LYSINE IN CORYNEBACTERIUM GLUTAMICUM BY COLORIMETRIC METHOD

Background and Aim:The use of microorganisms in production of economically and functionally valuable materials is taken into consideration in recent years. Amino acids are valuable substances that can be used in various industrial fields, including the pharmaceutical industry, healthcare products, food and feed. Lysine is essential amino acid in animal foods and used as a supplement to livestock, pigs and poultry.Methods:In this study, increasing the production of lysine by C. glutamicum was investigated and quantitative production checked by Colorimetric method .So carbon and nitrogen source to production of lysine by changing a factor method was optimized.Results:The results showed that the highest amount of lysine was produced in high fructose corn syrup as carbon source and ammonium sulfate as nitrogen source in 96 h incubation at pH=7,temptaure=30 °C and 150 rpm.Conclusion:Today, the biotechnology production amine acids due to their extensive usage is remarkable. By optimizing various factors in the production of them by microorganisms, production efficiency can be increased.

Keywords:

Optimization , Production , Lysine , Colorimetric method