STUDY OF THE EFFECT OF SILVER NANOPARTICLES ON GRAM NEGATIVE BACTERIA ISOLATED FROM RESISTANT URINARY TRACT INFECTION

Background and Aim:Urinary tract infection is one of the most common human infections. Due to antibiotic resistance, using a nanoparticle can eliminate a wide range of bacteria. When the silver becomes smaller in size to nanometres, silver increases its microbial properties, which is why it is important in medicine to investigate its effects.Methods:From 250 clinical specimens collected from the pathophysiological laboratory of Lahijan, 131 gram negative bacterial agents were isolated from UTI. The bacteria that were grown were identified by biochemical methods. Antimicrobial tests were performed according to the CLSI standard. The isolated gram negative bacteria were exposed to different concentrations (50 ppm, 200 ppm, 400 ppm) and the diameter of the non-growth hole was measured.Results:The results showed that E. coli were the most common bacteria responsible for UTI, with the frequency of 73.62%, Klebsiella 17.25%, Enterobacter 6.85%. Other isolates including Pseudomonas, Proteus, Citrobacter, providencia, Serratia and Morganella had the lowest incidence of 2.28%. All samples were sensitive to silver nanoparticles at concentrations of 100 ppm and 200 ppm. Enterobacter aerogenes was highest at 800 ppm concentration and Proteus vulgaris showed a lowest inhibition zone at 50 ppm.Conclusion:This study showed a direct correlation between the concentration of nanoparticles and the percentage of bacterial elimination, as the concentration of silver nanoparticles increased, more bacteria were lost.

Keywords:

UTI , Nanoparticles , Antibiotics resistance