INVESTIGATION THE SENSITIZATION OF ASTHMATIC PATIENTS TO A.ALTERNATA, BY IGE-IMMUNOBLOTTING

Background and Aim:There is evidence to demonstrate an association between fungal sensitization and asthma. Several studies have shown saprophytic fungi such as Alternaria, species are the most prevalent fungal allergens in the worldwide.Methods:Forty-eight patients with asthma (23 male, 25 female) and Forty-eight healthy controls (23 male, 25 female) were collected. Glass beads and liquid nitrogen was used to disrupted the cell wall of cultured fungi. SDS-PAGE was used to isolation of protein fractions. IgE immunoblotting against the patients and controls sera were performed to isolation of protein bands after electrotransfering into the nitrocellulose membrane.Results:Our results demonstrated the most allergenic bands consist to A. alternata with 17 bands (44.7%) and we found that asthmatic patients 41 to 70 years old were more sensitive when compared to others age groups.Conclusion:Our results showed that A. alternata had more power in sensitizing the patients.Also the protein bands with high molecular weight can be considered as an index of sensitizing in immunoblotting assay.

Keywords:

Asthma , Asthmatic patients , Alternaria alternate , IgE-immunoblotting