IN SILICO DESIGN OF A CHIMERIC HIGH IMMUNOGENIC OUTER MEMBRANE PROTEIN AS A NEW VACCINE CANDIDATE AGAINST SALMONELLA TYPHI

Publish Year: 1397
نوع سند: مقاله کنفرانسی
زبان: English
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شناسه ملی سند علمی:

MEDISM19_430

تاریخ نمایه سازی: 13 مهر 1397

Abstract:

Background and Aim:Enteric fever is a systemic infection caused by the Salmonella enterica serotype Typhi (S. Typhi) with a death rate of about 1%. The appearance of multidrug-resistant typhoid infections, highlights the necessity of control measures. Vaccination is an indispensable tool for the effective management of these kind of infections. With the limitations of existing S. Typhi vaccines, particularly their lack of effectiveness in young children, along with their limited availability in endemic countries, research to develop some new vaccines are needed. Previous works have shown that S. Typhi outer membrane proteins (OMPs) are highly immunogenic antigens. The aim of this study is in silico design a construct containing high immunogenic parts of two OMPs as a new vaccine candidate.Methods:Using bioinformatics tools, a new synthetic construct comprising outer membrane fimbrial usher proteins tsaC and steB is designed. The codon optimization of recombinant gene was carried out for a prokaryotic host. The mRNA structure of the gene and its stability and traslatibility were evaluated. The immunogenicity, B-cell epitope, allergenicity, and 3-D structure of chimeric protein with an appropriate linker were also determined.Results:In silico analyses showed that, two surface-exposed domains of selected OMPs with 569 amino acid were linked by a 4-repeated EAAAK linker. The construct was stable with high antigenicity, immunogenicity and solubility properties. Our in silico data showed that the fusion protein is not allergenic in the prokaryotic host.Conclusion:In conclusion, this new construct from S. Typhi OMPs could generate a potent immune response and can be experimentally studied as a vaccine candidate.

Authors

Ehsan Esmailnia

Department of Cell Biology, Faculty of Basic Sciences, Shahed University, Tehran, Iran

Seyed Latif Mousavi

Department of Cell Biology, Faculty of Basic Sciences, Shahed University, Tehran, Iran

Jafar Amani

Applied Microbiology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran