FREQUENCY OF CHLAMYDIA TRACHOMATIS, MYCOPLASMA GENITALIUM, UREAPLASMA UREALYTICUM AND LISTERIA MONOCYTOGENES ISOLATED FROM VAGINAL SAMPLES OF WOMEN IN KERMAN, IRAN

Background and Aim: Chlamydia trachomatis, Mycoplasma genitalium, Ureaplasma urealyticum and listeria monocytogenes are pathogens can pose serious complications during pregnancy and neonatal infection. Due to the importance of these bacteria and their lack of identification in Kerman, this study was conducted.Methods:This study was performed on 200 women who were referred to Afzalipour Hospital that ranged from 18 to 42 years old. 124 vaginal swab specimens were for aborted women and 76 cases for pregnant. Vaginal swab specimens were placed in two enrichment transport (TSB and yeast extract with and without antibiotics). Tubes containing antibiotic were placed in the refrigerator for L. monocytogenes, but another tubes used DNA extraction. Omp, mgpa, urease and inl j genes used for U. urealyticum, C. trachomatis, M. genitalium and L. monocytogenes. The enriched specimens were cultured on Blood agar and Palcam agar. The isolates were confirmed by specific phenotypic tests for L. monocytogenes.Results:PCR results showed that U. urealyticum, C. trachomatis and M. genitalium were higher in aborted women than in pregnant women so that 34.6% vs. 15.7% for U. urealyticum, 20.9% vs. 17.1% for M. genitalium and 15.3% vs. 10.5% for C. trachomatis. However, only for U. urealyticum the difference was significant (P= 0.0006). Cultures for L. monocytogenes were positive only about 5.5%, while PCR was positive by 29.5%.Conclusion:The present study showed high prevalence of U. urealyticum and L. monocytogenes in aborted and pregnant women, respectively. Diagnostic test is recommended to detect these bacteria in women with high risk.

Keywords:

Pregnant women , abortion , U. urealyticum , C. trachomatis , M. genitalium , L. monocytogenes