RAPID IDENTIFICATION OF SALMONELLA ENTERITIDIS IN CHICKEN SKIN USING INVA MOLECULAR MARKER

Background and Aim:Salmonella is a gram-negative that can cause diarrheal illness in humans. Raw meat, especially pork, undercooked products of poultry meat, eggs and products containing raw eggs as well as unpasteurized milk are foods posing the greatest hazard to public health. If present in food, it does not usually affect the taste, smell, or appearance of the food. The bacteria live in the intestinal tracts of infected animals and humans. In the present study, we employed RCR method for screening and identification of salmonella serovar Enteritidis in skins from commercial samples in Iran.Methods:Salmonella enterica serovars Enteritidis was grown on buffered peptone water. Artificial inoculation of chicken skin samples done. A control sample was also included to ensure that the skin was not naturally contaminated with Salmonella. DNA extracted from inoculated chicken skin samples. Amplification of the target sequence was performed using a PCR Express thermal cycler.Results:Using the PCR assay on genomic DNA from the chicken skin, artificially contaminated with S. Enteritidis, produced a band of 796 bp in size on electrophoresis. PCR assay on these skin samples resulted in 38 (71%) positive bands of 796 bp. Detected bands corresponding to 790 bp, represent contamination of the samples to the bacteria, with different rate depending on intensity of the bands.Conclusion:propounded the PCR assay evaluated in the current study could be used as a screening test, since results would be available in less time than with the cultural method.

Keywords:

Salmonella enteritidis , invA gene , PCR assay , commercial samples