SURVEY AND IDENTIFICATION OF ENTEROVIRUSES IN BOTTLED WATER BY PCR-RT METHOD

Publish Year: 1397
نوع سند: مقاله کنفرانسی
زبان: English
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MEDISM19_463

تاریخ نمایه سازی: 13 مهر 1397

Abstract:

Background and Aim:In view of water health and water borne diseases prevalence, Enterovirus 71 (EV71) infections, one of the major etiologic agents of hand, foot, and mouth disease, are considered as the most important public health concern in the Asia-Pacific region including Iran country. Enteroviruses are grouped into five types. The aim of this analytical study is to identify the Enterovirus contamination of bottled water in Tehran city.Methods:In this analytical study, random sampling is used. 22 samples are taken from different brands of bottled water of Tehran city during February to March 2018. The bottled water samples are transported in cold box and sterile condition according to procedure detailed in standard methods. Total virus numbers recovered are measured by quantitative reverse transcription-PCR (qRT-PCR).Results:The pH, temperature, and turbidity range of 22 samples are 7.2-7.8, 14-24 ̊C, and 0.4-1.3 NTU, respectively. The Enterovirus contamination range of 22 bottled water selected samples in Tehran city are 0 genome copies/L. The pH, and turbidity in all of samples present under than national standard values of bottled water.Conclusion:Bottled water is not sterile. This method is considered as the most performance in detecting Enterovirus in bottled water. We perform quantitative detection of Enterovirus in bottled water. The low num ber of samples is considered as our research limitation. It is concluded that supervision on bottled water production, transporting, and storage lead to prevent water viral contamination. Growth conditions play a critical role in the recovery of Enterovirus in bottled water.

Authors

Maryam Alamoti

Bachelor Student, Environmental Health, Islamic Azad University Tehran Medical Sciences Branch, Tehran, Iran

Giti Kashi

Associate professor, Department of Environmental Health, Islamic Azad University Tehran Medical Sciences Branch, Tehran, Iran Water Purification Research Centre (WPRC), Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran

Farbod Pazooki

Bachelor Student, Environmental Health, Islamic Azad University Tehran Medical Sciences Branch, Tehran, Iran