DETERMINATION OF 16 AND 18 GENOTYPES OF THE HUMAN PAPILLOMAVIRUS (HPV) IN CERVICAL CYTOLOGICAL SAMPLES FROM AHVAZ

Background and Aim:: Human papillomavirus is the main cause of cervical cancer. This cancer is the second most common cancer in women from worldwide especially in developing countries. Evidence of molecular epidemiology showed that the virus is associated with more than 90%of cancer tumore. So that HPV-16 and HPV-18 types include 70% of this .amount this study was prevalence with the aim of determination two high risk genotypes in cervical fluid cytology samples.Methods:In this study PCR was used to genotype determine. In this study was done on180 samples were collected from 16-72 old married women who referenced to the specialized women –clinics in year 1396. After screening of positive samples specific primers were used to two high risk types determinationResults:: In this study from180 samples HPV was detected in 33 specimens (%18/33).among this only one (%3/03) samples was detected as for the type 18 positive.Conclusion:: cervical cancer is easily diagnosed and treated at an early stages. But if its diagnosis delayed treatment will difficult and sometimes unsuccessful .Cervical screening has been reduced the incidence of cancer in developed countries. Therefore the effective prevention and treatment there is a need for adequate information about prevalence of HPV in each region.

Keywords:

HPV , PCR , genotype