CYTOTOXIC EFFECT OF SAPONINS EXTRACTED FROM SPIRULINA PLATENSIS ON THREE CANCER CELL LINES
Publish place: 19th International Congress of Microbiology of Iran
Publish Year: 1397
نوع سند: مقاله کنفرانسی
زبان: English
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شناسه ملی سند علمی:
MEDISM19_515
تاریخ نمایه سازی: 13 مهر 1397
Abstract:
Background and Aim:Many researches have emphasized on bioactive compounds extracted from microalgae. Spirulina platensis is an edible, filamentous, photosynthetic microalgae that has 25 kinds of vitamins and minerals and contains many compounds with biotic activity, such as alkaloids, phenolic compounds, terpenoids, saponins, … Saponins are polycyclic aglycones attached to one or more sugar side chains. Saponins mainly presence in plants and there are many studies about these compounds in plants, but there are few studies in S. platensis. This study aimed to estimate total saponin content in S. platensis and effect of this compound on cell viability in 3 cancer cell lines (HepG2, MCF-7, MDA-MB-123).Methods: Saponins were extracted using mixture of distilled water and n- butanol. Total saponin extracted were then dried and weighed. Cellular viability of HepG2, MCF-7 and MDA-MB-123 were evaluated using MTT assay after 24 h treatment with 0.02-2 mg/ml of saponins extracted from S. platensis.Results:Total saponin extracted from S. platensis was estimated 28± 0.0005 mg/g. TLC profiles showed four bands for saponins with Rf values of (0.44, 0.48, 0.50,0.55) with UV treatment 230 nm. IC50 values in HepG2, MCF-7, MDA-MB-123 cells obtained in 0.35 mg/ml, 0.4 mg/ml, 0.22 mg/ml respectively.
Keywords:
Saponin- Spirulina platensis- Anticancer
Authors
Mahboobeh Akbarizare
Iranian Research Organization for Science and Technology (IROST)
Hamideh Ofoghi
Iranian Research Organization for Science and Technology (IROST)
Mahnas Hadizadeh
Iranian Research Organization for Science and Technology (IROST)