STUDY THE ANTI-CANCER EFFECT OF BACTERIOCINS ISOLATED FROM LACTOBACILLUS-RHAMNOSUS ON CANCER CELL LINE AND ITS TOXICITY EFFECT ON NORMAL CELLS BY THE TRYPAN BLUE

Background and Aim:Bladder cancer is the second most common genital-urinary tract cancers in adults. On the other hand, the emergence of resistant tumor cells to chemotherapy medicines has required the discovery of new therapies. Bacteriocins are novel anti-cancer agents. In this study, we examine the anti-cancer activity of Bacteriocins lactobacillus-rhamnosus on the bladder cancer cells.Methods:We inoculated 2 × 10⁴ cell/ml of cancer cell line 5637 and normal HUVEC cell in 96-well plate and after 24 hours incubation, at 25, 50, 100, 200, 400 and 800 ppm concentrations Bacteriocins lactobacillus-rhamnosus was treated for 24 hours. The effect of Bacteriocins was measured by cell mortality tests. In cell mortality test, we used trypan blue solution and reverse microscope were used.Results:Bacteriocins inhibit the cancer cell 5637 growth and cause its death. Increasing the concentration of Bacteriocins is directly related to the increase in the death of cancer cells so that at the concentration of 800 ppm, the death of 71.43% of cancer cells is seen. But in normal cells, low toxicity is observed.Conclusion:Because of the changes in the surface of the cancer cell membrane relative to the normal cell, Bacteriocins can be easily linked to the cancer cell, and cause its death through induction of apoptosis. Therefore, Bacteriocins lactobacillus-rhamnosus can be used as an additional medicine and supplemental therapy for bladder cancer.

Keywords:

Bladder Cancer , Bacteriocins , lactobacillus-rhamnosus , Cell mortality