PRODUCTION AND FAST PARTIAL PURIFICATION OF STAPHYLOCOCCUS AUREUS SUPER ANTIGEN C

Background and Aim:Up to now, different methods have been described for the production and purification of bacterial toxins in native forms. In all these ways, Time consumption, high expensive equipment, high degree of specialty and costs were crucial. The purpose of this study was to provide rapid and inexpensive producer to semi- purifying the bacterial toxins.Methods:in this study, a standardize strain of S aureus with high level production of super antigen C was used. Mass production was lead to decanter precipitation and then centrifugation. The supernatant was subjected to sequential ultrafiltration 100, 50 30 1nd 10 Kda. The output was SDS- PAGE and confirmatory blotting.Results:The results revealed that, remaining 24- hour cultivation of mass production in decanter within 48 hours causes the participate of bacterial cells and proteins. The results of sequential ultrafiltration showed that the super antigen C ’is under ultra-filter 30KDa and were concentrated by ultra-filter 10KDa.Conclusion:Staphylococcal super antigen C is perhaps best recognized as the causative agents of food intoxications, although its role in other diseases such as inflammatory disorders is now being actively investigated. Clarification new hypothesis of this regards, it requires a native form of the toxins. The method used in this study would probably help to achieve these goals.

Keywords:

Production , purification , Staphylococcus aureus , super antigen C and ultrafiltration