STUDY OF CCR5-59353C/T POLYMORPHISM IN THE IRANIAN PATIENTS WITH CHRONIC HBV INFECTION

Publish Year: 1397
نوع سند: مقاله کنفرانسی
زبان: English
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شناسه ملی سند علمی:

MEDISM19_551

تاریخ نمایه سازی: 13 مهر 1397

Abstract:

Background and Aim:CCR5 is identified as one of the most important chemokine receptors which have a major role in the creation of chemotaxis and mobilization of immunocompetent cells and moving them to the liver for thorough cleaning of the virus. CCR5-59353 (C/T) is an important promoter polymorphism of chemokine receptor 5 gene. In some studies showed that there is a relationship between CCR5-59353 (C/T) polymorphism with clearance or persistence of HBV infection. The aim of this study was to evaluate polymorphism CCR5-59353 (C / T in Iranian patients with chronic HBV infection.Methods:A total of 200 blood samples including 100 healthy controls and 100 HBs Ag positive patients were randomly selected. Samples were tested for HBs Ag by ELISA and HBV-DNA by PCR method. Genomic DNA was extracted from blood buffy coat using the salting out method. CCR5-59353 (C/T) polymorphism was genotyped by allele specific amplification (ASA) PCR. Chi-square test was used for statistical analysis.Results:Five percent of control samples and Twelve percent of patient samples had CC mutant genotype. Nevertheless, there was no significant difference in genotypes frequency of CCR5-59353 between the two groups (P=0.1).Conclusion:It seems that CCR5-59353 polymorphism is not associated with chronic HBV infection outcome in Iranian population. However, frequency of CC genotype was higher in the patient group (12%) than control group (5%).

Authors

Farahnaz Bineshian

Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran,Iran

Zohreh Sharifi

Department of Parasitology & Mycology, Faculty of Medicine, Semnan University of Medical Sciences, Semnan, Iran

Sajjad Jalali

Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran,Iran