Mahdis Ghavidel - Microbiology and Virology Research Centre, Mashhad University of Medical Sciences, Mashhad, Iran. Department of Microbiology and virology, Mashhad University of Medical Sciences, Mashhad, Iran
Tahereh Golamhosseini Moghaddam - school of veterinary medicine, shiraz , Iran
Kimia Nourian - DVM, graduate student of school of veterinary medicine, Ferdowsi university of Mashhad, Mashhad, Iran
Kiarash Ghazvini - Microbiology and Virology Research Centre, Mashhad University of Medical Sciences, Mashhad, Iran. ۲. Department of Microbiology and virology, Mashhad University of Medical Sciences, Mashhad, Iran

Background and Aim:Urinary tract infections (UTIs) are one of the most common infectious diseases. Although different bacteria can be responsible for UTIs. E.coli is the most frequently isolated pathogen in UTIs. The pathogenic potential of E. coli strains dependent on the presence of virulence factors. The aim of this study was to determine the presence of main virulence genes by multiplex PCR and determine antibiotics resistance among clinical isolates in northeast of Iran.Methods:three hundred Escherichia coli isolates obtained from patients with urinary tract infection (UTI) referred to the Ghaem and Emam Reza Hospital different section, Mashhad, Iran. A Multiplex polymerase chain reaction (PCR) was used for the amplification of genes encoding pyelonephritis associated pili (pap genes), S-family adhesions (sfa gene), type1fimbriae (fimH gene), aerobactin (aer gene). The antibiotic susceptibility tests were done by the disk diffusion method. Different antibiotics were used such as B lactam, Aminoglycosides, Cephlosporine, Quinolone, Flurquinolone, Carbapenem and Trimethoprim-sulfamethoxazole.Results:The PCR results identified the fimH gene in (78.4%), aer in (70.5%), sfa gene in (13.6%) and the pap gene in (8.2%) of the isolates. The rate of antibiotic resistance of the strains is as follows: 64.7% resistant to cephalosporins, 34% to trimethoprim-sulfamethoxazole, 30.9% to fluoroquinolones, 15.2% to aminoglycosides, 13.2% to beta lactams 7.8% to quinolones and 4.4%. to carpabenems.Conclusion:in these study fimH and aer were found more than 50% UPEC strain isolated from patients that two gene have important role in pathogenesis. Major isolates have fimH as adhesion factor for colonization. More isolates resistance to cephalosporins cause of inappropriate administration.

UTI, E.coli, virulence factors, Multiplex PCR, Antibiotics resistance