Mahfam Sadri - Departmenr of Microbial Biotechlogy, Faculty of Basic Sciencces and Advanced Technologies in Biology, University of Science and Culture, Tehran, Iran
Naheed Mojgani - Department of Biotechnology, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization, Karaj, Iran
Nader Mosavari - Bovine Tuberculosis Reference Laboratory, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization, Karaj, Iran
Seyed Abolhassan Shahzade Fazeli - Departmenr of Microbial Biotechlogy, Faculty of Basic Sciencces and Advanced Technologies in Biology, University of Science and Culture, Tehran, Iran

Background and Aim:M.bovis is an important member of MTBC causing BT(bovine tuberculosis) in cattle and humans. This study aimed to We isolate and identify M.bovis strains from tuberculin positive cattle of Kerman province.Methods:Twenty five lymph nodes samples from BT suspected cattle from the Kerman province sent to Tuberculin reference Laboratory at Razi institute were analyzed. The isolates were initially decontaminated and cultivated on Lowenstein Jensen media with pyruvate and glycerin. Genomic DNA was isolated from pure colonies and subjected to 16srRNA and IS6110 primers for identification at Genus and Species level of the MTBC. The RFLP method determined the genomic pattern of M.bovis strains.Results:According to the obtained results, 10 samples appeared culture positive and were acid fast. Amplification of the genomic DNA by PCR-16srRNA and PCR-IS6110 showed that all the isolates belonged to the genus Mycobacterium and MTBC species. Based on RFLP typing and DNA hybridization results using the mentioned probes the M.bovis strains were determined and their DNA finger prints compared with the prevailing strains in the country. The strains specific to Kerman province were highlighted.Conclusion:The results confirms the presence of M.bovis infected cattle in Kerman province and indicates the possibility of the transfer to humans.

Mycobacterium bovis, RFLP typing, PCR-16srRNA, PCR-IS6110, PGRS and DR probes