EVALUATION OF THE ANTIMICROBIAL EFFECT OF THE ALCOHOLIC EXTRACT OF FLOWER, LEAVES BEFORE AND DURING FLOWERING OF CLERODENDRUM BUNGEI ON THE CLINICAL ISOLATES OF KLEBSIELLA SPP. , PSEUDOMONAS SPP. AND SHIGELLA SPP.

Background and Aim:The use of herbs for treatment of diseases has increased in recent years. Clerodendrum spp. is a plant of the Verbenaceae family. The aim of the present study was to investigate the antibacterial properties of alcoholic extract of Guilan native Clerodendrum bungei.Methods:Clinical isolates of Klebsiella spp., Pseudomonas spp. and Shigella spp. were collected from patients referred to Social welfare polyclinic in Rasht. After performing confirmatory diagnostic tests, alcoholic extracts of different parts of Clerodendrum bungei (leaves before and during flowering, flower) were prepared by maceration method and the antimicrobial activity of the extracts was evaluated using disk diffusion and broth dilution methods on 6 isolates of Klebsiella spp., Pseudomonas spp. and Shigella spp.Results:The results of the disc diffusion showed that the alcoholic extract of the leaves before flowering was more effective on Pseudomonas spp. than other bacteria. The MIC values of the different alcoholic extracts of Clerodendrum bungei on all isolates were 8.06 to 500 mg/ml. The MBC values of the different extracts of Clerodendrum bungei on isolates were 500 mg/ml, except for shigella spp., which was 250 mg/ml.Conclusion:It is concluded from the present study that Clerodendrum bungei possesses antibacterial properties.

Keywords:

Clerodendrum bungei , broth dilution , disk diffusion , klebsiella , pseudomonas , shigella