DETERMINATION OF ANTIBIOTIC RESISTANCE 1 PATTERN AND VIRULENCE GENES IN 2 ESCHERICHIA COLI ISOLATED FROM BOVINE WITH MASTITIS IN SOUTHWEST OF IRAN
Publish place: 19th International Congress of Microbiology of Iran
Publish Year: 1397
نوع سند: مقاله کنفرانسی
زبان: English
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شناسه ملی سند علمی:
MEDISM19_653
تاریخ نمایه سازی: 13 مهر 1397
Abstract:
Background and Aim:The aims of the present study were to investigate the prevalence of some virulence genes and also determine the antimicrobial resistance pattern of E. coli isolated from bovine with subclinical mastitis. The milk of 502 cows was collected from 8 dairy herd in the southwest of Iran.Methods:Conventional biochemical tests were used for identification of E. coli at the species level. Antimicrobial susceptibility patterns of E. coli isolates were determined by Disc Agar Diffusion method and Polymerase Chain Reaction (PCR) was used for detection of seven virulence genes including f17A, afaE-8, afaD-8, eaeA, cnf1, cnf2, and iucD. Seventy (13.94%) isolates of E. coli were identified in 502 milk samples.Results:The highest rate of resistance was observed against tetracycline (18.6%), while none of the isolates were resistant to streptomycin. Eight (11.5%) out of 70 E. coli isolates carried at least one of the virulence genes. The afaD-8 was the most prevalent gene detected in 5 (7.1%) isolates. The afaE-8, iucD, and eaeA detected in 3, 3 and 2 isolates respectivelyConclusion:Low prevalence of virulence factors may be indicating that most of the E. coli isolates originated from the commensal flora of cows and enter to the udders via environment contamination with feces.
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Authors
Masoud Marashifar
Student Research Committee, Yasuj University of Medical Sciences, Yasuj, Iran
Seyed Sajjad Khoramrooz
Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran
Zahra Karimii Aliabad
Department of Basic Sciences, Islamic Azad University, Yasooj Branch, Yasooj, Iran
Asghar Sharifi
Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran