Ladan Reisimotlagh - School of Public Health, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

Background and Aim:Brucellosis or brucellosis is one of the most well known and most important zoonoses in the world and especially in Iran. The consumption of milk and livestock products is one of the main ways of transmitting disease to humans. Horse milk is a rare and expensive product with higher nutrients than cow s milk. Due to the consumption of horse milk in infants and adolescents in Yazd, there is no information about the prevalence of brucellosis among animals, including mares of this city. The aim of this study was to determine the prevalence of brucellosis using serological methods and polymerase chain reaction in marijuana and marijuana in Yazd.Methods:In this study, 82 blood samples and 82 milk samples from the marijuana of Yazd clubs were collected in a sequential sampling. Serologic methods of Rose Bengal and ring testing were used. DNA was extracted directly from milk and blood samples. PCR test was performed using the primers ISP1 and ISP2 to detect Brucella genus using Ba-SP, Bm-SP and IS7 primers for detection of abortus and National Teniss species.Results:Based on serological tests no positive cases were found. By performing PCR to detect Brucella genus, 3 samples from milk samples were positive. All three positive specimens were identified as Brucella abortus.Conclusion:According to the findings of the study, the consumption of raw horses can cause a serious risk to the health of the community, thus revealing the need for health measures and information on horseradish milk.

Brucella,Raw milk , Blood, Horse, PCR